Journal: International Journal of Molecular Sciences
Article Title: Bioactivities of Alchemilla alpina L. Extract on Women’s Reproductive and Metabolic Health: Antioxidant, Enzyme Inhibitory, Receptor Modulatory Properties and Potential Cytotoxic Effects
doi: 10.3390/ijms27073025
Figure Lengend Snippet: Cytotoxicity of MeOH extract of Alchemilla alpina in a range of concentrations (6.25, 12.5, 25, 50 and 100 μg/mL) determined by the MTT assay in the ( A ) non-tumorigenic human fetal lung fibroblasts (MRC-5), ( B ) human cervical carcinoma cell line (HeLa) and ( C ) ovarian cancer cell line (A2780). Data represent the mean ± standard deviation (SD) (* p < 0.05, ** p < 0.01, and **** p < 0.0001 vs. Ctrl) by a one-way analysis of variance (ANOVA) with Tukey’s multiple comparison post hoc test, n = 9.
Article Snippet: Human fetal lung fibroblasts (MRC-5 and ATCC), human cervical carcinoma cells (HeLa and ATCC) and human ovarian cancer cells (A2780; a kind gift from Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia) were propagated in 25 cm 2 tissue culture flasks in a humidified incubator with 5% CO 2 at 37 °C.
Techniques: MTT Assay, Standard Deviation, Comparison